Coding

Part:BBa_K1489005:Design

Designed by: Iowis Zhu   Group: iGEM14_UMaryland   (2014-10-13)


Crasseoterra virginica Galectin 1 (CvGal1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 784
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 11
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 643


Design Notes

PstI site in the coding sequence had to be redesigned.


Source

Genome of Crasseoterra virginica, optimized for E. coli expression

References